Publications

Literature Reviews

Dr. Robert Murray, Professor Emeritus of Biochemistry at the University of Toronto, Canada, and Mannatech scientists review the literature regarding possible mechanisms of action for glyconutrients. 

Murray, R. K., Sinnott, R., Ramberg, J., Hall, J., Boyd, S. Recent dietary carbohydrates utilization research provides insights into the health effects of glyconutritional supplements. Presented at the Scripps Center for Integrative Medicine’s 3rd Annual Natural Supplements Conference, La Jolla, California. January 20-22, 2006. Download Research PDF

Ambrotose^® product constituents

Basic Science

Dr. John Kalns and colleagues (Hyperion Biotechnology, Inc., San Antonio, TX) developed an in vitro method that showed promise in predicting the immunostimulatory properties of different aloe vera gel preparations.*

Kalns, J., Kirchner, J., Ebong, S., Oubre, C. (2007) A sensitive in vitro method for evaluation of immunostimulatory properties of natural products. Presented at the Scripps Center for Integrative Medicine’s 4th Annual Natural Supplements Conference, La Jolla, California. January 19-21, 2007. Download Research PDF

Bonakdar, R. A. (2007) Report on the 4th Annual Natural Supplements Research Competition. J Alt Comp Med 13, 686-87.

Mannatech scientists conducted this study, showing that the processing of aloe vera gel significantly impacts its saccharide composition and molecular weight and particle size distributions. These changes, which likely affect product quality and efficacy, suggest that dietary supplement GMPs will not adequately control aloe gel product quality.

Duncan, C., Ramberg, J., Sinnott, R. Striking differences in aloe vera gel carbohydrate composition, molecular weight and particle size distributions following processing will not be addressed by dietary supplement GMPs. Presented at the Scripps Center for Integrative Medicine’s 5th Annual Natural Supplements Conference, San Diego, California. January 17-20, 2008. Download Research PDF

Mannatech scientists conducted this test tube study to better understand the breakdown of Ambrotose^® products’ constituent polysaccharides in the gut. They reported which GI tract bacterial glycosylhydrolases could break down aloe vera powder and Undaria pinnatifida extract (in Advanced Ambrotose^® products), aloe vera extract (in Ambrotose^® complex), and larch arabinogalactan, gum ghatti and gum tragacanth (in both Ambrotose^® products). 

Duncan CL, Luta G, Ramberg J, Zhang TX, Sinnott RA (2009) Intestinal microbiota's naturally occurring enzymes and their effects on plant polysaccharide digestion. Presented at the Scripps Center for Integrative Medicine's 6th Annual Natural Supplements Conference, San Diego, California. January 22-25, 2009. Download Research PDF

Mannatech sponsored this research, conducted by outside experts, to determine the chemical profile (MW and total carbohydrate and protein content) and carbohydrate composition (total sugars and free sugars) of aloe vera powder, aloe vera extract, larch arabinogalactan, and Undaria pinatifida extract. 

Luta G, Duncan C, Sinnott R (2009) Chemical characterization of polysaccharide-rich ingredients from Aloe vera, Larix laricina and Larix occidentalis, and Undaria pinatifida. Presented at the Scripps Center for Integrative Medicine's 6th Annual Natural Supplements Conference, San Diego, California. January 22-25, 2009. Download Research PDF

Ambrotose^® products

In Vitro

Dr. John Kalns and colleagues (Hyperion Biotechnology, Inc., San Antonio, TX) conducted an in vitro study demonstrating that human fecal bacteria can partially break down Advanced Ambrotose^® powder and two of its constituents (aloe vera gel polysaccharides and arabinogalactan). Bacteria identified that could best compete for these polysaccharides were Enterococcus species (a species that is a popular probiotic in some countries).

Sinnott RA, Kalns JE, Boyd S, Ramberg J, Duncan C, Oubre C, Fitzpatrick A, Geistweidt J, Kirchner JM (2007) Human colonic bacteria can utilize the large polysaccharides of aloe vera gel and arabinogalactan polysaccharides as their only carbon source. Presented at the 107th American Society for Microbiology Meeting, Toronto, Canada. May 21-25, 2007. Download Research PDF

Sinnott RA, Ramberg J, Kirchner JM, Oubre C, Duncan C, Boyd S, Kalns JE (2007) Utilization of arabinogalactan, aloe vera gel polysaccharides, and a mixed saccharide dietary supplement by human colonic bacteria in vitro . Int J Probiotics Prebiotics 2, 97-104.

Bonakdar, R. A. (2007) Report on the 4th Annual Natural Supplements Research Competition. J Alt Comp Med 13, 686.

Human Interventional: Double-Blind, Placebo-Controlled Studies

Drs. Chenghua Wang (Rotman Research Institute, Toronto, Canada), Joanne Szabo (University of Arkansas for Medical Sciences, Little Rock, ARK) and Roscoe Dykman (Arkansas Children’s Nutrition Center, Little Rock, ARK) showed, in a study of 20 healthy adults, that a one tablespoon serving of Ambrotose^® complex powder enhanced brainwave frequencies known to be associated with attention or alertness.*

Wang, C., Szabo, J. S., Dykman, R. A. (2004) Effects of a carbohydrate supplement upon resting brain activity. Integr Physiol Behav Sci 39, 126-138. Download Research PDF

Drs. Chenghua Wang and R.T. Pivik (University of Arkansas, Little Rock, ARK) showed, in a study of 20 healthy adults, that a one tablespoon serving of Ambrotose^® complex powder improved mental reaction time and enhanced concentration.*

Wang, C., Pivik, R. T., Dykman, R. A. Effects of a glyconutritional supplement on brain potentials associated with language processing. Federation Proceedings: Experimental Biology Meeting, New Orleans, Louisiana. April 20-24, 2002. Download Research PDF

Dr. Talitha Best (Flinders University, Adelaide, Australia) showed that, in a trial of 45 middle-aged male and female subjects, a single 7 gram serving of Ambrotose^® complex powder improved memory.* (Note: this serving is 1.75 times the recommended dose [4 grams/day] for this product).

Best T (2005) An investigation of saccharide effects on memory performance. Abstracts of the 12th Annual Australian Psch Conf 57, 41-78.

Dr. Talitha Best (Flinders University, Adelaide, Australia) showed, in a trial of 109 healthy middle-aged male and female adults, that Ambrotose^® complex powder (4 grams/day for 12 weeks) significantly improved memory and improved psychological well-being.*

Best T, Kemps E, Bryan J. Saccharide effects on cognition and well-being in middle-aged adults: A randomized controlled trial. Developmental Neuropsychology 2010; 35(1): 66 -80. Download Research PDF

Drs. Atiya N. Stancil and Leslie H. Hicks, both of Howard University in Washington, D.C., published this randomized, double-blind, placebo-controlled study investigating the impact of Ambrotose^® complex on the brain function of 62 healthy young adults. The study showed that a single, one-tablespoon serving of Ambrotose complex significantly improved visual discrimination and working memory.

Stancil AN, Hicks LH. Glyconutrients and perception, cognition, and memory. Perceptual Mot Skills 2009;10:259-70.

Ambrotose AO^® capsules

Human Interventional: Clinical Trial

Mannatech scientists and an independent statistician showed, in pilot study of 12 healthy adults, that 2 capsules/day Ambrotose AO^® capsules increased serum ORAC (a measure of antioxidant protection) by 37.4% compared with baseline (before supplementation) serum ORAC values.*

Boyd, S., Gary, K., Koepke, C. M., McAnalley, S., Ford, C. R., Horn, E., McAnalley, B. H. (2003) An open-label pilot study of the antioxidant effect in healthy people of Ambrotose AO . GlycoScience & Nutrition 4, 1-6. Download Research PDF

BounceBack™ capsules

Human Interventional: Randomized, Double-Blind, Placebo-controlled Crossover Trial

Dr. Jay Udani (UCLA Geffen School of Medicine, Los Angeles, California and Medicus Research LLC, Northridge, California), and Drs. Betsy Singh, Vijay Singh and Elizabeth Sandoval (Medicus Research LLC) showed that 10 healthy adults experienced reduced pain and tenderness following eccentric exercise after 30 days of supplementation with BounceBack™ capsules (2 /day).*

Udani J, Singh B, Singh V, Sandoval E (2008) Effect of a combination dietary supplement product (BounceBack) on the signs and symptoms of delayed onset muscle soreness after eccentric exercise: a randomized, double-blind, placebo-controlled, crossover pilot study. Poster presentation at the 5th Annual International Society of Sports Nutrition Conference, June 8-10, 2008, Las Vegas, Nevada. Download Mannatech Research PDF

Udani,J., Singh,B., Singh,V. (2009). BounceBack capsules for reduction of DOMS after eccentric exercise: a randomized, double-blind, placebo-controlled, crossover pilot study. J Int Soc Sports Nutr, 6, 14. Download Research PDF

EMPACT™ Sports Drink

Human Interventional: Double-Blind, Placebo-Controlled Studies

Dr. Allyn Byars (Angelo State University, San Angelo, TX), Drs. Mike and Lori Greenwood (Baylor University, Waco, TX) and Dr. Warren K. Simpson (Hardin-Simmons University, Abilene, TX) showed that, when consumed by healthy subjects instead of water prior to exercise, EMPACT™ sports drink enhanced indices of cardiorespiratory fitness (specifically VO_2max and time to exhaustion).*

Byars, A., Greenwood, M. G. L. (2006) The effectiveness of a pre-exercise performance drink (PRX) on indices of maximal cardiorespiratory fitness. J Int Soc Sports Nutr 3, 56-59. Download Research PDF

Dr. Allyn Byars, Kelly D. Schneider, Mandy C. Hesseltine (Angelo State University ), Dr. Warren K. Simpson (Hardin-Simmons University, Abilene, TX), and Dr. Mike Greenwood (Baylor University, Waco, TX) EM-PACT consumed prior to exercise, enhanced aerobic performance compared to the nation’s leading sports drink in healthy subjects.*

Schneider, K., Hessletine, M., Greenwood, M. A comparison of two sport drinks on aerobic performance. Presented at the Annual International Society's Sports & Nutrition Conference, June 15-17. 2006.

Byars, A., Schneider, K. D., Hesseltine, M. C., Simpson, W. K., Greenwood, M. (2007) Sports nutrition: comparing two sports drinks on aerobic performance. Appl Res Coach Athletics Ann 22, 226-240.

Dr. Byars and colleagues showed that a modified version of EMPACT™ sports drink (without creatine monohydrate—to meet the compliance standards set forth by various athletic organizations that regulate the use of nutritional supplements) significantly improved maximal oxygen consumption (VO_2max), time to exhaustion and percentage estimated non-protein fat substrate utilization when consumed by healthy subjects instead of water prior to a treadmill test.*

Byars A, Keith S, Snowden S (2009) The influence of a pre-exercise sports drink on indices of aerobic power. Presented at the Scripps Center for Integrative Medicine's 6th Annual Natural Supplements Conference, San Diego, California. January 22-25, 2009.
Download Research PDF

OsoLean™ powder

Open-label Human Trial

In a study conducted by Mannatech scientists and an independent statistician, 8 weeks of OsoLean™ powder consumption significantly increased weight loss and waist inches lost, significantly decreased BMI and appetite, and improved quality of life.*

Sinnott RA, Maddela RL, Nelson ED, Bae S, Singh KP, Anderson JA (2009) Preliminary study on the effects of a calcium-rich whey protein supplement (OsoLean™ powder) on weight loss, waist circumference, and appetite in overweight subjects. Presented at the Scripps Center for Integrative Medicine's 6th Annual Natural Supplements Conference, San Diego, California. January 22-25, 2009.
Download Research PDF

Sinnott RA, Maddela RL, Nelson ED, Bae S, Singh KP, Anderson JA. The modifying effects of a calcium-rich whey protein supplement (OsoLean™ Powder) on weight loss and waist circumference in overweight subjects: a preliminary study. Open Nutraceuticals J 2009; 2:36-41. Download Research PDF

Randomized, double-blind, parallel arm study

Drs. Joy Frestedt and John Zenk (Minnesota Applied Research Center, Edina, MN), Michael Kuskowski (Geriatric Research Education and Clinical Center, Minneapolis, MN), and Loren Ward and Eric Bastian (Glanbia Research and Development Center, Twin Falls, ID) conducted this 12-week study on healthy subjects on a diet that reduced caloric intake by 500 calories/day. They showed that subjects who consumed a flavored and sweetened drink mix that contained the same peptide blend in OsoLean™ powder lost significantly more body fat and lost less lean muscle mass when compared with those consuming an isocaloric beverage.*

Frestedt JL, Zenk JL, Kuskowski MA, Ward LS, Bastian ED. A whey-protein supplement increases fat loss and spares lean muscle in obese subjects: a randomized human clinical study. Nutr Metab (Lond) 2008; 5:8.

 
 

---